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验证数据展示

  • IHC staining of mouse kidney using 24676-1-AP

    IHC staining of mouse kidney using 24676-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24676-1-AP (ATP13A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 24676-1-AP

    IHC staining of mouse kidney using 24676-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24676-1-AP (ATP13A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24676-1-AP

    IHC staining of mouse brain using 24676-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24676-1-AP (ATP13A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 24676-1-AP

    IF Staining of rat brain using 24676-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ATP13A5 antibody (24676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP13A5 Polyclonal antibody

ATP13A5 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 24676-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, ELISA

别名

ATPase type 13A5, P5 ATPase isoform 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
Biotin
规格价格库存

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经过测试的应用

Positive IHC detected inmouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24676-1-AP targets ATP13A5 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20337

Product name: Recombinant human ATP13A5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1123-1218 aa of BC156652

Sequence: TQFCVAFFVEDSILQNHELWLLIKREFGFYSKSQYRTWQKKLAEDSTWPPINRTDYSGDGKNGFYINGGYESHEQIPKRKLKLGGQPTEQHFWARL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase type 13A5
别名 ATPase type 13A5, P5 ATPase isoform 5
计算分子量 1218 aa, 137 kDa
GenBank蛋白编号BC156652
基因名称 ATP13A5
Gene ID (NCBI) 344905
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4VNC0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP13A5 is a member of the P5 subfamily of P-type transport ATPases. ATP13A5 functions within the intracellular membrane system and is mainly responsible for maintaining the ionic homeostasis and phospholipid balance of the intracellular membrane system.

实验方案

Product Specific Protocols
IF protocol for ATP13A5 antibody 24676-1-APDownload protocol
IHC protocol for ATP13A5 antibody 24676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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