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验证数据展示

  • WB analysis of mouse brain using 19141-1-AP

    WB analysis of mouse brain using 19141-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 19141-1-AP

    IHC staining of mouse brain using 19141-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 19141-1-AP

    IHC staining of mouse brain using 19141-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PARK9/ATP13A2 Polyclonal antibody

PARK9/ATP13A2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19141-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

ATP13A2, ATPase type 13A2, HSA9947, KRPPD, PARK9, PARK9/ATP13A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19141-1-AP targets PARK9/ATP13A2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 PARK9/ATP13A2 fusion protein Ag11435 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase type 13A2
别名 ATP13A2, ATPase type 13A2, HSA9947, KRPPD, PARK9, PARK9/ATP13A2
计算分子量 1158 aa, 126 kDa
观测分子量125-130 kDa
GenBank蛋白编号BC030267
基因名称 ATP13A2
Gene ID (NCBI) 23400
RRIDAB_2918064
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ11
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP13A2 belongs to the P-type superfamily of ATPases that transport inorganic cations and other substrates across cell membranes. It appears to protect cells from manganese and zinc toxicity, possibly by causing cellular efflux and/or lysosomal sequestration; and from iron toxicity, possibly by preserving lysosome integrity against iron-induced lipid peroxidation (21724849)(24334770). However, it potentiates the toxic effects of cadmium and nickel on developing neurites, and of the widely used herbicide paraquat possibly by increasing polyamine uptake. Deficiency is associated with spastic paraplegia and Kufor-Rakeb syndrome, in which there is progressive parkinsonism with dementia.

实验方案

Product Specific Protocols
WB protocol for PARK9/ATP13A2 antibody 19141-1-APDownload protocol
IHC protocol for PARK9/ATP13A2 antibody 19141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols