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验证数据展示

  • WB analysis of HEK-293 using 86762-3-RR (same clone as 86762-3-PBS)

    WB analysis of HEK-293 using 86762-3-RR (same clone as 86762-3-PBS)

    37°C incubated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86762-3-RR (ATP13A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

  • WB analysis of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    WB analysis of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    37°C incubated mouse liver tissue were subjected to SDS PAGE followed by western blot with 86762-3-RR (ATP13A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    IHC staining of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86762-3-RR (ATP13A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP13A1 antibody included
  • IHC staining of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    IHC staining of mouse liver using 86762-3-RR (same clone as 86762-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86762-3-RR (ATP13A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATP13A1 antibody included
  • IF Staining of HeLa using 86762-3-RR (same clone as 86762-3-PBS)

    IF Staining of HeLa using 86762-3-RR (same clone as 86762-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATP13A1 antibody (86762-3-RR, Clone: 251765A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86762-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86762-3-RR

    Affinity and Kinetic Characterization of 86762-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86762-3-RR against Human ATP13A1 were performed. The affinity constant is 0.629 nM.

ATP13A1 Recombinant monoclonal antibody, PBS Only

ATP13A1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86762-3-PBS

产品说明书

CloneNo. 251765A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ATP13A, CGI-152, EC:7.4.2.-, Endoplasmic reticulum P5A-ATPase, Endoplasmic reticulum transmembrane helix translocase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ATP13A1 Ready-To-Use IHC Kit. ATP13A1 primary antibody included.

产品信息

86762-3-PBS targets ATP13A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9174

Product name: Recombinant human ATP13A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 615-758 aa of BC009302

Sequence: QKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPTLSNSGIRATSRTAKQRSGLPPSEEQPTSQRDRLSQVLRDLEDESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCTLVTTLQM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase type 13A1
别名 ATP13A, CGI-152, EC:7.4.2.-, Endoplasmic reticulum P5A-ATPase, Endoplasmic reticulum transmembrane helix translocase
计算分子量 976 aa, 109 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC009302
基因名称 ATP13A1
Gene ID (NCBI) 57130
RRIDAB_3745107
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9HD20
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP13A1 is a P-type ATPase, mainly involved in the transport of cations within cells. It plays an important role in maintaining intracellular ion homeostasis and membrane potential. ATP13A1 acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane (PubMed:32973005).

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