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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83785-6-RR (same clone as 83785-6-PBS)

    WB analysis using 83785-6-RR (same clone as 83785-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83785-6-RR (ATOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 83785-6-RR (same clone as 83785-6-PBS)

    FC experiment of PC-3 using 83785-6-RR (same clone as 83785-6-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83785-6-RR (same clone as 83785-6-PBS)

    FC experiment of HeLa using 83785-6-RR (same clone as 83785-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATOX1 Recombinant antibody (83785-6-RR, Clone:240679E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83785-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83785-6-RR

    Affinity and Kinetic Characterization of 83785-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83785-6-RR against Human ATOX1 were performed. The affinity constant is 183 nM.

    {{ptg:ProductFeaturesInfo}}

ATOX1 Recombinant monoclonal antibody, PBS Only

ATOX1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83785-6-PBS

产品说明书

CloneNo. 240679E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

ATX1, ATOX 1, Antioxidant 1, 240679E9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ATOX1 Ready-To-Use IHC Kit. ATOX1 primary antibody included.

产品信息

83785-6-PBS targets ATOX1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18460

Product name: Recombinant human ATOX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-68 aa of BC112248

Sequence: MPKHEFSVDMTCGGCAEAVSRVLNKLGGVKYDIDLPNKKVCIESEHSMDTLLATLKKTGKTVSYLGLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATX1 antioxidant protein 1 homolog (yeast)
别名 ATX1, ATOX 1, Antioxidant 1, 240679E9
计算分子量 68 aa, 8 kDa
观测分子量 7 kDa
GenBank蛋白编号BC112248
基因名称 ATOX1
Gene ID (NCBI) 475
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO00244
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Antioxidant 1 (ATOX1) is a copper chaperone to regulate copper homeostasis in cell. In the cytosol, ATOX1 always binds to copper and transfer to ATPase proteins in the trans-Golgi network, thereby promoting copper supply to various copper-dependent oxidereductases matured within the secretory vesicles (PMID: 28294521). Also, the protein could protect cells against oxidative damage and the expression levels of ATOX1 may help to the inflammatory response and antioxidant defense,even to modulate response to the cancer (PMID: 27472369). ATOX1 plays an important role in the physiology of the human central nervous system ,and it's highly expressed in the cerebral cortex and hippocampus (PMID: 30545441). Either cytosol or nucleus location of ATOX1 has been reported in different literations, which may be associated with cell status (PMID: 24445997; 31317143).