验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, fetal human brain tissue, pig brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31924-1-AP targets ATL1 in WB, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat, pig |
| 免疫原 | ATL1 fusion protein Ag29223 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | atlastin GTPase 1 |
| 别名 | Brain-specific GTP-binding protein, Atlastin-1, atlastin1, atlastin GTPase 1, Atlastin 1 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC010708 |
| 基因名称 | ATL1 |
| Gene ID (NCBI) | 51062 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8WXF7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATL1 encodes atlastin-1, a GTPase that plays a crucial role in maintaining the structure and function of the endoplasmic reticulum. Mutations in ATL1 are one of the most common causes of hereditary spastic paraplegia (HSP) (PMID: 38851544). tlastin-1 is regulated by ubiquitination, a post-translational modification that can affect its activity and localization. For example, the E3 ubiquitin ligase SYVN1 has been shown to ubiquitinate atlastin-1, inhibiting its GTPase activity and thereby affecting ER morphology (PMID: 32916628).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATL1 antibody 31924-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |