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ATG7 Recombinant antibody
ATG7 Recombinant Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2M8
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, U2OS cells, Jurkat cells |
Positive IP detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81760-1-RR targets ATG7 in WB, IP, ELISA applications and shows reactivity with Human samples.
Tested Applications | IP, WB, ELISA |
Tested Reactivity | Human |
Immunogen | ATG7 fusion protein Ag0142 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | ATG7 autophagy related 7 homolog (S. cerevisiae) |
Synonyms | APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7 |
Calculated Molecular Weight | 78 kDa |
Observed Molecular Weight | 68-78 kDa |
GenBank Accession Number | BC000091 |
Gene Symbol | ATG7 |
Gene ID (NCBI) | 10533 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O95352 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for ATG7 antibody 81760-1-RR | Download protocol |
IP protocol for ATG7 antibody 81760-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |