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验证数据展示

  • WB analysis of Jurkat using 20382-1-AP

    WB analysis of Jurkat using 20382-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20382-1-AP (ATG4C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 20382-1-AP

    IHC staining of human heart using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 20382-1-AP

    IHC staining of human heart using 20382-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20382-1-AP (ATG4C antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 20382-1-AP

    IF Staining of HeLa using 20382-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG4C antibody (20382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ATG4C Polyclonal antibody

ATG4C Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 20382-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF, IHC, ELISA

别名

APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20382-1-AP targets ATG4C in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag14207

Product name: Recombinant human ATG4C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC033024

Sequence: MEATGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDEDKTLPAESGCTIEDHVIAGNVEEFRKDFISRIWLTYREEFPQIEGSALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALNIENSDSESWTSHTVKKFTASFEASLSGEREFKTPTISLKETIGKYSDDHEMRNEVYHRKIISWFGDSPLALFGLHQLIEYGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNADDKAVIILVPVRLGGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG4 autophagy related 4 homolog C (S. cerevisiae)
别名 APG4 C, APG4C, ATG4C, AUTL1, AUTL3, Autophagin 3, Cysteine protease ATG4C
计算分子量 458 aa, 52 kDa
观测分子量 52-55 kDa
GenBank蛋白编号BC033024
基因名称 ATG4C
Gene ID (NCBI) 84938
RRIDAB_10666852
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DT6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATG4C antibody 20382-1-APDownload protocol
IHC protocol for ATG4C antibody 20382-1-APDownload protocol
IF protocol for ATG4C antibody 20382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Autophagy

Analysis of ATG4C function in vivo

Authors - Isaac Tamargo-Gómez
View Article
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun
View Article