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验证数据展示

  • WB analysis using 23226-1-AP (same clone as 23226-1-PBS)

    WB analysis using 23226-1-AP (same clone as 23226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23226-1-AP (ATG2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 23226-1-AP (same clone as 23226-1-PBS)

    IF Staining of MCF-7 using 23226-1-AP (same clone as 23226-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATG2A antibody (23226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 23226-1-AP (same clone as 23226-1-PBS)

    IP experiment of K-562 using 23226-1-AP (same clone as 23226-1-PBS)

    IP result of anti-ATG2A (IP:23226-1-AP, 4ug; Detection:23226-1-AP 1:4000) with K-562 cells lysate 2400 ug. This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 23226-1-AP (same clone as 23226-1-PBS)

    IHC staining of mouse kidney using 23226-1-AP (same clone as 23226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATG2A antibody included
  • IHC staining of mouse kidney using 23226-1-AP (same clone as 23226-1-PBS)

    IHC staining of mouse kidney using 23226-1-AP (same clone as 23226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23226-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATG2A antibody included
  • Featured Product
  • KD/KO Validated

ATG2A Polyclonal antibody, PBS Only

ATG2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 23226-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

KIAA0404, Autophagy-related protein 2 homolog A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ATG2A Ready-To-Use IHC Kit. ATG2A primary antibody included.

产品信息

23226-1-PBS targets ATG2A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19709

Product name: Recombinant human ATG2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-539 aa of BC110650

Sequence: RVQRLEYCDEAVRDPSQAPPVDVHQPPAFLHKLLQLAGVRLHYEELPAQEEPPEPPLQIGSCSGYMELMVKLKQNEAFPGPKLEVAGQLGSLHLLLTPRQLQQLQELLSAVSLTDHEGLADKLNKSRPLGAEDLWLIEQDLNQQLQAGAVAEPLSPDPLTNPLLNLDNTDLFFSMAGLTSSVASALSELSLSDVDLASSVRSDMASRRLSAQAHPAGKMAPNPLLDTMRPDSLLKMTLGGVTLTLLQTSAPSSGPPDLATHFFTEFDATKDGPFGSRDFHHLRPRFQRACPCSHVRLTGTAVQLSWELRTGSRGRRTTSMEVHFGQLEVLECLWPRGTSEPEYTEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG2 autophagy related 2 homolog A (S. cerevisiae)
别名 KIAA0404, Autophagy-related protein 2 homolog A
计算分子量 1938 aa, 213 kDa
观测分子量 215 kDa
GenBank蛋白编号BC110650
基因名称 ATG2A
Gene ID (NCBI) 23130
RRIDAB_2879235
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ2TAZ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Autophagy-related protein 2 homolog A (ATG2A) belongs to the ATG2 family. ATG2A is a lipid transfer protein involved in autophagosome assembly (PMID:31271352). ATG2A tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. ATG2A binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PMID: 30952800, PMID: 31271352). ATG2A also regulates lipid droplet morphology and distribution within the cell (PMID: 28561066). The observed MW of ATG2A is 215 kDa.

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