验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
24322-1-AP targets ATG16L2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag19399 Product name: Recombinant human ATG16L2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 17-365 aa of BC137490 Sequence: HIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALRVKWQEEEEGLRLVCGEMAYQVVEKGAALGTLESELQQRQSRLAALEARVAQLREARAQQAQQVEEWRAQNAVQRAAYEALRAHVGLREAALRRLQEEARDLLERLVQRKARAAAERNLRNERRERAKQARVSQELKKAAKRTVSISEGPDTLGDGMRERRETLALAPEPEPLEKEACEKWKRPFRSASATSLTLSHCVDVVKGLLDFKKRRGHSIGGAPEQRYQIIPVCVAARLPTRAQDVLDAHLSEVNAVRFGPNSSLLATGGADRLIHLWNV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATG16 autophagy related 16-like 2 (S. cerevisiae) |
| 别名 | APG16 like 2, ATG16L2, Autophagy related protein 16 2, WDR80 |
| 计算分子量 | 619 aa, 69 kDa |
| 观测分子量 | 70 kDa, 30 kDa |
| GenBank蛋白编号 | BC137490 |
| 基因名称 | ATG16L2 |
| Gene ID (NCBI) | 89849 |
| RRID | AB_2879493 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NAA4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| IP protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| WB protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
