验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, HT-1376 cells, K-562 cells, TF-1 cells, HSC-T6 cells, RAW 264.7 cells |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68813-4-Ig targets ATF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag34935 Product name: Recombinant human ATF1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-271 aa of BC029619 Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | activating transcription factor 1 |
| 别名 | cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36 |
| 计算分子量 | 271 aa, 29 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC029619 |
| 基因名称 | ATF1 |
| Gene ID (NCBI) | 466 |
| RRID | AB_3743314 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P18846 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ATF1 antibody 68813-4-Ig | Download protocol |
| WB protocol for ATF1 antibody 68813-4-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
