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验证数据展示

  • WB analysis using 13973-1-AP

    WB analysis using 13973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13973-1-AP (ATE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13973-1-AP

    WB analysis of HeLa using 13973-1-AP

    WB result of ATE1 antibody (13973-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATE1 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

ATE1 Polyclonal antibody

ATE1 Polyclonal Antibody for WB, ELISA
Cat No. 13973-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

arginyltransferase 1, ATE1, R transferase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, HeLa cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13973-1-AP targets ATE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATE1 fusion protein Ag5006 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 arginyltransferase 1
别名 arginyltransferase 1, ATE1, R transferase 1
计算分子量 59 kDa
观测分子量 59-65 kDa
GenBank蛋白编号BC022026
基因名称 ATE1
Gene ID (NCBI) 11101
RRIDAB_2060763
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95260
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginyltransferases (ATE1s) are eukaryotic enzymes that catalyze the non-ribosomal, post-translational addition of the amino acid arginine to an acceptor protein. ATE1 plays critical roles in many biological functions including cardiovascular development, angiogenesis, adipogenesis, muscle contraction, and metastasis of cancer.

实验方案

Product Specific Protocols
WB protocol for ATE1 antibody 13973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols