验证数据展示
经过测试的应用
| Positive WB detected in | MAF cells |
| Positive IP detected in | NCCIT cell |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16610-1-AP targets ATAD3A/B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ATAD3A/B fusion protein Ag9836 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase family, AAA domain containing 3B |
| 别名 | AAA TOB3, ATAD3A, ATAD3B, KIAA1273, TOB3 |
| 计算分子量 | 72 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC002542 |
| 基因名称 | ATAD3B |
| Gene ID (NCBI) | 83858 |
| RRID | AB_2878288 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T9A4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties. This antibody can detect both ATAD3A and ATAD3B.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATAD3A/B antibody 16610-1-AP | Download protocol |
| IP protocol for ATAD3A/B antibody 16610-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis | ||
Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA | ||
EMBO J ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.
| ||
Oxid Med Cell Longev miRNA-27a Transcription Activated by c-Fos Regulates Myocardial Ischemia-Reperfusion Injury by Targeting ATAD3a.
| ||
Int J Mol Sci Analysis of Radiation Toxicity in Mammalian Cells Stably Transduced with Mitochondrial Stat3 | ||
J Cell Sci ATAD3 controls mitochondrial cristae structure, influencing mtDNA replication and cholesterol levels in muscle.
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