验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23894-1-AP targets ATAD2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag20947 Product name: Recombinant human ATAD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC113656 Sequence: MVVLRSSLELHNHSAASATGSLDLSSDFLSLEHIGRRRLRSAGAAQKKPAATTAKAGDGSSVKEVETYHRTRALRSLRKDAQNSSDSSFEKNVEITEQLANGRHFTRQLARQQADKKKEEHREDKVIPVTRSLRARNIVQSTEHLHEDNG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATPase family, AAA domain containing 2 |
| 别名 | ANCCA, ATAD2, PRO2000 |
| 计算分子量 | 1390 aa, 159 kDa |
| 观测分子量 | 159 kDa |
| GenBank蛋白编号 | BC113656 |
| 基因名称 | ATAD2 |
| Gene ID (NCBI) | 29028 |
| RRID | AB_2879352 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6PL18 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATAD2 is one of them encoding a conserved factor harbouring an AAA type ATPase domain and a bromodomain. ATAD2 controls chromatin dynamics, genome transcriptional activities and apoptotic cell response. This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ATAD2 antibody 23894-1-AP | Download protocol |
| IP protocol for ATAD2 antibody 23894-1-AP | Download protocol |
| WB protocol for ATAD2 antibody 23894-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Proteomic profiling reveals distinct phases to the restoration of chromatin following DNA replication | ||
Biomed Pharmacother Silencing METTL3 inhibits the proliferation and invasion of osteosarcoma by regulating ATAD2. | ||
Mol Cell Biol Mutual Balance of Histone Deacetylases 1 and 2 and the Acetyl Reader ATAD2 Regulates the Level of Acetylation of Histone H4 on Nascent Chromatin of Human Cells.
| ||
Biochem Biophys Res Commun ATAD2 drives colorectal cancer progression by regulating TRIM25 expression via a positive feedback loop with E2F transcriptional factors.
| ||
iScience Genome-wide survey of D/E repeats in human proteins uncovers their instability and aids in identifying their role in the chromatin regulator ATAD2 |
