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验证数据展示

  • WB analysis using 84252-4-RR

    WB analysis using 84252-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84252-4-RR

    IF Staining of HeLa using 84252-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84252-4-RR

    Affinity and Kinetic Characterization of 84252-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84252-4-RR against Human ASUN were performed. The affinity constant is below 1 pM.

ASUN Recombinant monoclonal antibody

ASUN Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84252-4-RR

产品说明书

CloneNo. 241586E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASUN Ready-To-Use IHC Kit. ASUN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84252-4-RR targets ASUN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13727

Product name: Recombinant human C12orf11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 357-706 aa of BC003081

Sequence: GRSVLLEQPRKSGSKVISHMLSSHGGEIFLHVLSSSRSILEDPPSISEGCGGRVTDYRITDFGEFMRENRLTPFLDPRYKIDGSLEVPLERAKDQLEKHTRYWPMIISQTTIFNMQAVVPLASVIVKESLTEEDVLNCQKTIYNLVDMERKNDPLPISTVGTRGKGPKRDEQYRIMWNELETLVRAHINNSEKHQRVLECLMACRSKPPEEEERKKRGRKREDKEDKSEKAVKDYEQEKSWQDSERLKGILERGKEELAEAEIIKDSPDSPEPPNKKPLVEMDETPQVEKSKGPVSLLSLWSNRINTANSRKHQEFAGRLNSVNNRAELYQHLKEENGMETTKNGKASRQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 12 open reading frame 11
别名 INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11
计算分子量 706 aa, 80 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC003081
基因名称 ASUN
Gene ID (NCBI) 55726
RRIDAB_3671801
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NVM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).

实验方案

Product Specific Protocols
IF protocol for ASUN antibody 84252-4-RRDownload protocol
WB protocol for ASUN antibody 84252-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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