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验证数据展示

  • IF Staining of MCF-7 using 66036-1-Ig (same clone as 66036-1-PBS)

    IF Staining of MCF-7 using 66036-1-Ig (same clone as 66036-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody 66036-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 66036-1-Ig (same clone as 66036-1-PBS)

    WB analysis of pig liver using 66036-1-Ig (same clone as 66036-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

    IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASS1 antibody included
  • IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

    IHC staining of human liver using 66036-1-Ig (same clone as 66036-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66036-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASS1 antibody included
  • Featured Product
  • KD/KO验证

ASS1 Monoclonal antibody, PBS Only

ASS1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66036-1-PBS

产品说明书

CloneNo. 1D1A11

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ASS1 Ready-To-Use IHC Kit. ASS1 primary antibody included.

产品信息

66036-1-PBS targets ASS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 ASS1 fusion protein Ag9314 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 argininosuccinate synthetase 1
别名 Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1
计算分子量 412 aa, 47 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC009243
基因名称 ASS1
Gene ID (NCBI) 445
RRIDAB_11042883
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00966
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).