验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, ARPE-19 cells, Jurkat cells, K-562 cell |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human retinoblastoma tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14681-1-AP targets ASNS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6393 Product name: Recombinant human ASNS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 247-561 aa of BC014621 Sequence: LMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | asparagine synthetase |
| 别名 | Asparagine synthetase [glutamine-hydrolyzing], EC:6.3.5.4, Glutamine-dependent asparagine synthetase, TS11 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 55-64 kDa |
| GenBank蛋白编号 | BC014621 |
| 基因名称 | ASNS |
| Gene ID (NCBI) | 440 |
| RRID | AB_2060119 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08243 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ASNS antibody 14681-1-AP | Download protocol |
| IHC protocol for ASNS antibody 14681-1-AP | Download protocol |
| IP protocol for ASNS antibody 14681-1-AP | Download protocol |
| WB protocol for ASNS antibody 14681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase. | ||
Cell Metab Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.
| ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
| ||
Mol Cell Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
| ||
Nat Commun p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.
| ||
J Exp Med Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer. |
