• Featured Product
  • KD/KO Validated

ASNS Polyclonal antibody

ASNS Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14681-1-AP

Print datasheet

Synonyms

ASNS, asparagine synthetase, TS11



经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman retinoblastoma tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14681-1-AP targets ASNS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, IP, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen ASNS fusion protein Ag6393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asparagine synthetase
Synonyms ASNS, asparagine synthetase, TS11
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 55-64 kDa
GenBank Accession NumberBC014621
Gene Symbol ASNS
Gene ID (NCBI) 440
RRIDAB_2060119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08243
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

实验方案

Product Specific Protocols
WB protocol for ASNS antibody 14681-1-APDownload protocol
IHC protocol for ASNS antibody 14681-1-APDownload protocol
IF protocol for ASNS antibody 14681-1-APDownload protocol
IP protocol for ASNS antibody 14681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Metab

ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.

Authors - Robert T Williams
humanWB

Cell Metab

Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.

Authors - Abigail S Krall
  • KO Validated
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
  • KD Validated
humanWB, IHC

Mol Cell

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Authors - Ji Zhang
  • KD Validated
humanWB

Nat Commun

p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.

Authors - Longfei Deng
  • KD Validated
humanIHC

J Exp Med

Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.

Authors - Maria Victoria Recouvreux