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ASMTL Polyclonal antibody

ASMTL Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14981-1-AP

Print datasheet

Synonyms

ASMTL, ASMTLX, ASMTLY, ASTML

验证数据展示

  • WB analysis of human testis using 14981-1-AP

    WB analysis of human testis using 14981-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14981-1-AP

    IHC staining of human prostate cancer using 14981-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14981-1-AP (ASMTL antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human prostate cancer using 14981-1-AP

    IHC staining of human prostate cancer using 14981-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14981-1-AP (ASMTL antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of A549 using 14981-1-AP

    IF Staining of A549 using 14981-1-AP

    Immunofluorescent analysis of A549 cells using 14981-1-AP (ASMTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman testis tissue, A549 cells, HeLa cells, HL-60 cells, L02 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14981-1-AP targets ASMTL in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen ASMTL fusion protein Ag6894 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acetylserotonin O-methyltransferase-like
Synonyms ASMTL, ASMTLX, ASMTLY, ASTML
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69-75 kDa
GenBank Accession NumberBC002508
Gene Symbol ASMTL
Gene ID (NCBI) 8623
RRIDAB_2059989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95671
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ASMTL antibody 14981-1-APDownload protocol
IHC protocol for ASMTL antibody 14981-1-APDownload protocol
IF protocol for ASMTL antibody 14981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols