验证数据展示
经过测试的应用
| Positive WB detected in | COS-7 cells, COLO 320 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12331-1-AP targets ASH2L in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, monkey samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, ChIP |
| 经测试反应性 | human, mouse, monkey |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3008 Product name: Recombinant human ASH2L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 234-534 aa of BC015936 Sequence: HGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPEDTETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYKDIFEGVYFPAISLYKSCTVSINFGPCFKYPPKDLTYRPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ash2 (absent, small, or homeotic)-like (Drosophila) |
| 别名 | ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2 |
| 计算分子量 | 628 aa, 69 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC015936 |
| 基因名称 | ASH2L |
| Gene ID (NCBI) | 9070 |
| RRID | AB_2059837 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UBL3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ASH2L antibody 12331-1-AP | Download protocol |
| IP protocol for ASH2L antibody 12331-1-AP | Download protocol |
| WB protocol for ASH2L antibody 12331-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation. | ||
Cell Rep Nuclear-localized HKDC1 promotes hepatocellular carcinoma through phosphorylating RBBP5 to upregulate H3K4me3 | ||
Cancer Sci ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription.
| ||
Mol Neurobiol WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling. |
