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ASGR1 Monoclonal antibody

ASGR1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G5F5

Cat No : 66692-1-Ig

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Synonyms

ASGP R 1, ASGPR, ASGPR 1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056



经过测试的应用

Positive WB detected inHuH-7 cells, L02 cells, HepG2 cells, pig liver tissue, mouse hepatocytes, HSC-T6 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66692-1-Ig targets ASGR1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Pig, rat samples.

Tested Applications IF, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Pig, rat
Immunogen ASGR1 fusion protein Ag2310 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name asialoglycoprotein receptor 1
Synonyms ASGP R 1, ASGPR, ASGPR 1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight 42-46 kDa
GenBank Accession NumberBC032130
Gene Symbol ASGR1
Gene ID (NCBI) 432
RRIDAB_2882046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

实验方案

Product Specific Protocols
WB protocol for ASGR1 antibody 66692-1-IgDownload protocol
IHC protocol for ASGR1 antibody 66692-1-IgDownload protocol
IF protocol for ASGR1 antibody 66692-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols