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验证数据展示

  • WB analysis using 31338-1-AP (same clone as 31338-1-PBS)

    WB analysis using 31338-1-AP (same clone as 31338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31338-1-AP (ASGR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    IHC staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IHC staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    IHC staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IF Staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    IF Staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (31338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

  • IF Staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    IF Staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (31338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

ASGR1 Polyclonal antibody, PBS Only

ASGR1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31338-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.

产品信息

31338-1-PBS targets ASGR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asialoglycoprotein receptor 1
别名
计算分子量33 kDa
观测分子量38-40 kDa
GenBank蛋白编号NM_001671.4
基因名称 ASGR1
Gene ID (NCBI) 432
RRIDAB_3669951
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07306-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

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