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验证数据展示

  • WB analysis using 85848-1-RR (same clone as 85848-1-PBS)

    WB analysis using 85848-1-RR (same clone as 85848-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85848-1-RR (ASF1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85848-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02155-2

    Cytometric bead array standard curve of MP02155-2

    Cytometric bead array standard curve of MP02155-2, ASF1B-specific Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85848-3-PBS. Detection antibody: 85848-1-PBS. Standard: Ag17763. Range: 0.781-50 ng/mL.

ASF1B Recombinant antibody, PBS Only (Detector)

ASF1B Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 85848-1-PBS

产品说明书

CloneNo. 250195A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

ASF1A/B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, CIA II, CIA-II

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ASF1B Ready-To-Use IHC Kit. ASF1B primary antibody included.

产品信息

85848-1-PBS targets ASF1B as part of a matched antibody pair:

MP02155-2: 85848-3-PBS capture and 85848-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ASF1B fusion protein Ag17763 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
别名 ASF1A/B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, CIA II, CIA-II
计算分子量 202 aa, 22 kDa
观测分子量19 kDa
GenBank蛋白编号BC010014
基因名称 ASF1B
Gene ID (NCBI) 55723
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NVP2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.