验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22258-1-AP targets ASF1B-specific in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17763 Product name: Recombinant human ASF1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-202 aa of BC010014 Sequence: INWDNNMDRLEAIETQDPSLGCGLPLNCTPIKGLGLPGCIPGLLPENSMDCI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ASF1 anti-silencing function 1 homolog B (S. cerevisiae) |
| 别名 | ASF1A/B, ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, CIA II |
| 计算分子量 | 202 aa, 22 kDa |
| 观测分子量 | 20-22 kDa |
| GenBank蛋白编号 | BC010014 |
| 基因名称 | ASF1B |
| Gene ID (NCBI) | 55723 |
| RRID | AB_2879051 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NVP2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody specifically reacts with the 19kd ASF1B protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ASF1B-specific antibody 22258-1-AP | Download protocol |
| IHC protocol for ASF1B-specific antibody 22258-1-AP | Download protocol |
| WB protocol for ASF1B-specific antibody 22258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.
| ||
Epigenetics Chromatin Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice. | ||
Cancer Lett Activation of the FOXM1/ASF1B/PRDX3 axis confers hyperproliferative and antioxidative stress reactivity to gastric cancer | ||
Int J Biol Macromol Tannic acid protects against colitis by regulating the IL17 - NFκB and microbiota - methylation pathways | ||
Tissue Cell ASF1B is an essential prognostic indicator linked to the growth and resistance characteristics of bladder cancer | ||
