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验证数据展示

  • WB analysis using 22259-1-AP

    WB analysis using 22259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 22259-1-AP

    IF Staining of A431 using 22259-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ASF1A-specific antibody (22259-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

ASF1A-specific Polyclonal antibody

ASF1A-specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 22259-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

ASF1A, Anti-silencing function protein 1 homolog A, CGI-98, CGI 98, CCG1-interacting factor A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22259-1-AP targets ASF1A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag17764

Product name: Recombinant human ASF1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 151-204 aa of BC010878

Sequence: INWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
别名 ASF1A, Anti-silencing function protein 1 homolog A, CGI-98, CGI 98, CCG1-interacting factor A
计算分子量 204 aa, 23 kDa
观测分子量 20-23 kDa
GenBank蛋白编号BC010878
基因名称 ASF1A
Gene ID (NCBI) 25842
RRIDAB_2879052
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y294
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. This antibody is specific to the 23kd ASF1A protein.

实验方案

Product Specific Protocols
IF protocol for ASF1A-specific antibody 22259-1-APDownload protocol
WB protocol for ASF1A-specific antibody 22259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.

Authors - Sumin Feng
  • KD Validated
View Article
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