验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HL-60 cells, U-937 cells, THP-1 cells |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human colon cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88328-1-RR targets ASC/TMS1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag0774 Product name: Recombinant human TMS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC004470 Sequence: MGRARDAILDALENLTAEELKKFKLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | PYD and CARD domain containing |
| 别名 | PYCARD, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5, Caspase recruitment domain-containing protein 5 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 15-22 kDa |
| GenBank蛋白编号 | BC004470 |
| 基因名称 | ASC/TMS1 |
| Gene ID (NCBI) | 29108 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9ULZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9. TMS1/ASC has different isoforms: ASC, ASC-b and ASC-c (PMID: 20482797).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ASC/TMS1 antibody 88328-1-RR | Download protocol |
| IP protocol for ASC/TMS1 antibody 88328-1-RR | Download protocol |
| WB protocol for ASC/TMS1 antibody 88328-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
