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验证数据展示

  • WB analysis using 24031-1-AP

    WB analysis using 24031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 24031-1-AP

    WB analysis of mouse skeletal muscle using 24031-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24031-1-AP

    IF Staining of HepG2 using 24031-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASB5 antibody (24031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

ASB5 Polyclonal antibody

ASB5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24031-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ASB-5, ASB 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24031-1-AP targets ASB5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21230

Product name: Recombinant human ASB5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 245-329 aa of BC065710

Sequence: STEIVNLLLEFGADINAKNTELLRPIDVATSSSMVERILLQHEATPSSLYQLCRLCIRSYIGKPRLHLIPQLQLPTLLKNFLQYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat and SOCS box-containing 5
别名 ASB-5, ASB 5
计算分子量 329 aa, 36 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC065710
基因名称 ASB5
Gene ID (NCBI) 140458
RRIDAB_2879410
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWX0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASB5, also named as Ankyrin repeat and SOCS box-containing 5, is a 329 amino acid protein, which contains a conserved one C-terminal SOCS box motif. Asb5 was significantly upregulated in growing collateral arteries on mRNA and protein level. It has been shown that asb5 is a protein implicated in the initiation of arteriogenesis.

实验方案

Product Specific Protocols
IF protocol for ASB5 antibody 24031-1-APDownload protocol
WB protocol for ASB5 antibody 24031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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