验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, mouse testis tissue, rat skeletal musle tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33262-1-AP targets ASB12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag36991 Product name: Recombinant human ASB12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 12-161 aa of BC069436 Sequence: LLQPDKEEEDTDTEEKQALNQAVYDNDSYTLDQLLRQERYKRFINSRSGWGVPGTPLRLAASYGHLSCLQVLLAHGADVDSLDVKAQTPLFTAVSHGHLDCVRVLLEAGASPGGSIYNNCSPVLTAARDGAVAILQELLDHGAEANVKAK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ankyrin repeat and SOCS box-containing 12 |
| 别名 | Ankyrin repeat and SOCS box protein 12, ASB-12 |
| 计算分子量 | 309 aa, 34 kDa/318 aa, 35 kDa |
| 观测分子量 | 34,39 kDa |
| GenBank蛋白编号 | BC069436 |
| 基因名称 | ASB12 |
| Gene ID (NCBI) | 142689 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8WXK4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ankyrin repeat and SOCS box (ASB) family is composed of 18 proteins from ASB1 to ASB18 and belongs to the suppressor of cytokine signaling (SOCS) box protein superfamily. ASB family proteins interact with Cul5-Rbx2 to form E3 Ub ligases and play significant roles via a ubiquitination-mediated pathway (PMID: 16325183). The calculated molecular weight of ASB12 in mice or rat was 34 kda. ASB12 may be ubiquitinated, resulting in an increase in the observed molecular quantity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASB12 antibody 33262-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
