ASAP3 Polyclonal antibody
ASAP3 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, L02 cells |
Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
14099-1-AP targets ASAP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IHC, WB, ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | ASAP3 fusion protein Ag5231 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 |
Synonyms | ACAP4, ASAP3, CENTB6, DDEFL1, UPLC1 |
Calculated Molecular Weight | 903 aa, 99 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC060786 |
Gene Symbol | ASAP3 |
Gene ID (NCBI) | 55616 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8TDY4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ASAP3 antibody 14099-1-AP | Download protocol |
IHC protocol for ASAP3 antibody 14099-1-AP | Download protocol |
IF protocol for ASAP3 antibody 14099-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Aging (Albany NY) Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes. |