验证数据展示
经过测试的应用
| Positive WB detected in | mouse small intestine tissue |
Planning an IHC experiment? We recommend our IHCeasy ASAH2 Ready-To-Use IHC Kit. ASAH2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
27742-1-AP targets ASAH2 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag26908 Product name: Recombinant human ASAH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 105-333 aa of BC107105 Sequence: GVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 |
| 别名 | Acylsphingosine deacylase 2, ASAH2, hCD, HNAC1, LCDase, N CDase, NCDase, Neutral ceramidase, Non lysosomal ceramidase |
| 计算分子量 | 780 aa, 86 kDa |
| 观测分子量 | 100-115 kDa |
| GenBank蛋白编号 | BC107105 |
| 基因名称 | ASAH2 |
| Gene ID (NCBI) | 56624 |
| RRID | AB_2918129 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR71 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASAH2 antibody 27742-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Prev Res (Phila) Stool Protein Mass Spectrometry Identifies Biomarkers for Early Detection of Diffuse-type Gastric Cancer | ||
Life Metab High temperature ameliorates high-fat diet-induced obesity by promoting ceramide breakdown in skeletal muscle tissue |
