ASAH1 Polyclonal antibody
ASAH1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, SW480 cells, T-47D cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy ASAH1 Ready-To-Use IHC Kit. ASAH1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11274-1-AP targets ASAH1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ASAH1 fusion protein Ag1799 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | N-acylsphingosine amidohydrolase (acid ceramidase) 1 |
| Synonyms | AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, HSD 33, PHP, PHP32, Putative 32 kDa heart protein |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 37-40 kDa |
| GenBank Accession Number | BC016828 |
| Gene Symbol | ASAH1 |
| Gene ID (NCBI) | 427 |
| RRID | AB_2058719 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13510 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). The predicted molecular weight of ASAH1 is 45 kDa. ASAH1 is a glycoprotein processed from a 55-60 kDa precursor via autoproteolytic cleavage into a mature heterodimeric enzyme formed by an α-subunit (13 kDa) and a β-subunit (37-40 kDa) (PMID: 22261821).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASAH1 antibody 11274-1-AP | Download protocol |
| IHC protocol for ASAH1 antibody 11274-1-AP | Download protocol |
| IF protocol for ASAH1 antibody 11274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease. | ||
Hum Mol Genet Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function. | ||
Int J Mol Sci Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation. | ||
J Dermatol Sci Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis. | ||
Arch Dermatol Res A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum. |
