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验证数据展示

  • WB analysis of mouse testis using 87108-1-RR (same clone as 87108-1-PBS)

    WB analysis of mouse testis using 87108-1-RR (same clone as 87108-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 87108-1-RR (ART3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87108-1-PBS in a different storage buffer formulation.

  • FC experiment of OVCAR-3 using 87108-1-RR (same clone as 87108-1-PBS)

    FC experiment of OVCAR-3 using 87108-1-RR (same clone as 87108-1-PBS)

    1x10^6 OVCAR-3 cells were intracellularly stained with 0.25 ug ART3 Recombinant monoclonal antibody (87108-1-RR, Clone:252232A3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 87108-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 87108-1-RR (same clone as 87108-1-PBS)

    IHC staining of human heart using 87108-1-RR (same clone as 87108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 87108-1-RR (ART3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87108-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 87108-1-RR (same clone as 87108-1-PBS)

    IHC staining of human heart using 87108-1-RR (same clone as 87108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 87108-1-RR (ART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87108-1-PBS in a different storage buffer formulation.

  • Affinity of 87108-1-RR

    Affinity of 87108-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87108-1-RR against Human ART3 were performed. The affinity constant is 0.205 nM.

ART3 Recombinant monoclonal antibody, PBS Only

ART3 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 87108-1-PBS

产品说明书

CloneNo. 252232A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

ADP ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, EC:2.4.2.31, Ecto ADP ribosyltransferase 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87108-1-PBS targets ART3 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8775

Product name: Recombinant human ART3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-364 aa of BC008397

Sequence: EVLDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVWENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAFHFYLTRALQLLRKPCEASSKTVVYRTSQGTSFTFGGLNQARFGHFTLAYSAKPQAANDQLTVLSIYTCLGVDIENFLDKESERITLIPLNEVFQVSQEGAGNNLILQSINKTCSHYECAFLGGLKTENCIENLEYFQPIYVYNPGEKNQKLEDHSEKNWKLEDHGEKNQKLEDHGVKILEPTQIPGMKIPEPFPLPEDKSQGNINNPTPGPVPVPGPKSHPSASLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ADP-ribosyltransferase 3
别名 ADP ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, EC:2.4.2.31, Ecto ADP ribosyltransferase 3
计算分子量 389 aa, 44 kDa
观测分子量38-47 kDa
GenBank蛋白编号BC008397
基因名称 ART3
Gene ID (NCBI) 419
RRIDAB_3745345
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13508
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ART3 is a GPI-anchored mono-ADP-ribosyltransferase enriched on the sperm cell surface, where it participates in spermatid maturation and membrane remodeling. Localized to lipid rafts, ART3 likely modulates cell-cell signaling and extracellular protein modification. Its upregulation in multiple cancers suggests additional roles in migration, invasion, and tumor aggressiveness. Despite incomplete characterization of its enzymatic activity, ART3 remains a key regulator at the interface of reproductive biology, membrane signaling, and pathology.

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