验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
25060-1-AP targets ARRDC3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag18717 Product name: Recombinant human ARRDC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 14-122 aa of BC053619 Sequence: DCLNDSNVPVYSSGDTVSGRVNLEVTGEIRVKSLKIHARGHAKVRWTESRNAGSNTAYTQNYTEEVEYFNHKDILIGHERDDDNSEEGFHTIHSGRHEYAFSFELPQTP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | arrestin domain containing 3 |
| 别名 | arrestin domain containing 3, Arrestin domain-containing protein 3, TBP-2-like inducible membrane protein, TLIMP |
| 计算分子量 | 414 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC053619 |
| 基因名称 | ARRDC3 |
| Gene ID (NCBI) | 57561 |
| RRID | AB_2879878 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96B67 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARRDC3 (Arrestin domain-containing protein 3) is an adaptor protein involved in modulating the cell-surface abundance of adrenergic receptors and likely other G protein-coupled receptors. It participates in NEDD4-dependent ubiquitination, endocytosis, and subsequent degradation of activated ADRB2. ARRDC3 may recruit NEDD4 to ADRB2; alternatively, it may act as an adaptor that contributes to endosomal targeting of ADRB2 rather than serving as a primary mediator for NEDD4 recruitment to this receptor (PMID: 20559325; 21982743; 23208550).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARRDC3 antibody 25060-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
