验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HL-60 cells, K-562 cells, HeLa cells, HepG2 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84874-5-RR targets ARRDC1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ARRDC1 fusion protein Ag15341 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | arrestin domain containing 1 |
| 别名 | 242396A5, Alpha-arrestin 1, arrestin domain containing 1, Arrestin domain-containing protein 1 |
| 计算分子量 | 433 aa, 46 kDa |
| 观测分子量 | 46-50 kDa |
| GenBank蛋白编号 | BC032346 |
| 基因名称 | ARRDC1 |
| Gene ID (NCBI) | 92714 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N5I2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARRDC1, or Arrestin-Domain Containing Protein 1, is a protein that plays a significant role in the formation and release of extracellular vesicles, specifically a subtype known as ARMMs (Arrestin-Domain Containing Protein 1-mediated microvesicles). ARRDC1 is localized to the cytosolic side of the plasma membrane and recruits the ESCRT-I complex protein TSG101 to initiate outward membrane budding. This protein is also involved in the regulation of protein cargo and release of extracellular vesicles, including both exosomes and ectosomes. The absence of ARRDC1 can lead to changes in the protein cargo within these vesicles, indicating its importance in the sorting and packaging process.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARRDC1 antibody 84874-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |