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验证数据展示

  • WB analysis using 21030-1-AP (same clone as 21030-1-PBS)

    WB analysis using 21030-1-AP (same clone as 21030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21030-1-AP (ARRDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21030-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 21030-1-AP (same clone as 21030-1-PBS)

    IHC staining of mouse kidney using 21030-1-AP (same clone as 21030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21030-1-AP (ARRDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21030-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 21030-1-AP (same clone as 21030-1-PBS)

    IHC staining of mouse kidney using 21030-1-AP (same clone as 21030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21030-1-AP (ARRDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21030-1-PBS in a different storage buffer formulation.

ARRDC1 Polyclonal antibody, PBS Only

ARRDC1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21030-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

Alpha-arrestin 1, arrestin domain containing 1, Arrestin domain-containing protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21030-1-PBS targets ARRDC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15341

Product name: Recombinant human ARRDC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 84-433 aa of BC032346

Sequence: HSFPFQFLLPATAPTSFEGPFGKIVHQVRAAIHTPRFSKDHKCSLVFYILSPLNLNSIPDIEQPNVASATKKFSYKLVKTGSVVLTASTDLRGYVVGQALQLHADVENQSGKDTSPVVASLLQKVSYKAKRWIHDVRTIAEVEGAGVKAWRRAQWHEQILVPALPQSALPGCSLIHIDYYLQVSLKAPEATVTLPVFIGNIAVNHAPVSPRPGLGLPPGAPPLVVPSAPPQEEAEAEAAAGGPHFLDPVFLSTKSHSQRQPLLATLSSVPGAPEPCPQDGSPASHPLHPPLCISTGATVPYFAEGSGGPVPTTSTLILPPEYSSWGYPYEAPPSYEQSCGGVEPSLTPES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 arrestin domain containing 1
别名 Alpha-arrestin 1, arrestin domain containing 1, Arrestin domain-containing protein 1
计算分子量 433 aa, 46 kDa
观测分子量46-50 kDa
GenBank蛋白编号BC032346
基因名称 ARRDC1
Gene ID (NCBI) 92714
RRIDAB_3085633
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5I2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARRDC1, or Arrestin-Domain Containing Protein 1, is a protein that plays a significant role in the formation and release of extracellular vesicles, specifically a subtype known as ARMMs (Arrestin-Domain Containing Protein 1-mediated microvesicles). ARRDC1 is localized to the cytosolic side of the plasma membrane and recruits the ESCRT-I complex protein TSG101 to initiate outward membrane budding. This protein is also involved in the regulation of protein cargo and release of extracellular vesicles, including both exosomes and ectosomes. The absence of ARRDC1 can lead to changes in the protein cargo within these vesicles, indicating its importance in the sorting and packaging process.

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