验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, U-937 cells, rat lung tissue, mouse lung tissue, RAW 264.7 cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue, mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15361-1-AP targets Beta Arrestin 1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7608 Product name: Recombinant human Arrestin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 69-418 aa of BC003636 Sequence: DVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGKACGVDYEVKAFCAENLEEKIHKRNSVRLVIRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQYADICLFNTAQYKCPVAMEEADDTVAPSSTFCKVYTLTPFLANNREKRGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPKEEPPHREVPENETPVDTNLIELDTNDDDIVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | arrestin, beta 1 |
| 别名 | ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1, β-Arrestin-1 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47-55 kDa |
| GenBank蛋白编号 | BC003636 |
| 基因名称 | Beta Arrestin 1 |
| Gene ID (NCBI) | 408 |
| RRID | AB_2060227 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49407 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Beta Arrestin 1 antibody 15361-1-AP | Download protocol |
| IP protocol for Beta Arrestin 1 antibody 15361-1-AP | Download protocol |
| WB protocol for Beta Arrestin 1 antibody 15361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Autonomous sensing of the insulin peptide by an olfactory G protein-coupled receptor modulates glucose metabolism.
| ||
J Cell Biol BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone. | ||
Cell Death Dis β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy. | ||
J Biol Chem An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectors.
| ||
PLoS One Cell Survival Following Radiation Exposure Requires miR-525-3p Mediated Suppression of ARRB1 and TXN1. | ||
Oncol Rep Blue light irradiation inhibits the growth of colon cancer and activation of cancer‑associated fibroblasts. |
