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ARPP-19 Polyclonal antibody

ARPP-19 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11678-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

ARPP19, cAMP-regulated phosphoprotein 19, ARPP16, ARPP 19, ARPP 16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inJurkat cells, A375 cells, A549 cells, mouse brain tissue, rat skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy ARPP19 Ready-To-Use IHC Kit. ARPP19 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11678-1-AP targets ARPP-19 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ARPP-19 fusion protein Ag2279 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclic AMP phosphoprotein, 19 kD
别名 ARPP19, cAMP-regulated phosphoprotein 19, ARPP16, ARPP 19, ARPP 16
计算分子量 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC003418
基因名称 ARPP-19
Gene ID (NCBI) 10776
RRIDAB_2060078
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP56211
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARPP-19, a 19-kDa cAMP-regulated phosphoprotein, plays a role in regulating mitosis. Initiation and maintenance of mitosis require the activation of protein kinase cyclin B-Cdc2 and the inhibition of protein phosphatase 2A (PP2A). When phosphorylated by protein kinase Greatwall (Gwl), ARPP-19 associate with and inhibit PP2A, thus promoting mitotic entry. ARPP-19 may be an important link between nerve growth factor (NGF) signaling and post-transcriptional control of neuronal gene expression such as GAP-43.

实验方案

Product Specific Protocols
WB protocol for ARPP-19 antibody 11678-1-APDownload protocol
IHC protocol for ARPP-19 antibody 11678-1-APDownload protocol
IF protocol for ARPP-19 antibody 11678-1-APDownload protocol
IP protocol for ARPP-19 antibody 11678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Authors - Xigang Xia
humanWB

J Proteome Res

Phosphoproteome Profiling Revealed the Importance of mTOR Inhibition on CDK1 Activation to Further Regulate Cell Cycle Progression.

Authors - Luqi Jin
humanWB,IHC

Onco Targets Ther

PMID: 32753897

Authors - Xiang Gao
  • KD Validated
humanWB

Int J Mol Sci

Increased ARPP-19 expression is associated with hepatocellular carcinoma.

Authors - Haiyan Song
humanWB

Cancers (Basel)

Arpp19 Promotes Myc and Cip2a Expression and Associates with Patient Relapse in Acute Myeloid Leukemia.

Authors - Eleonora Mäkelä
  • KD Validated
humanWB,IP

Nature

Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19

Authors - Sathish K R Padi
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