验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse small intestine tissue, rat lymph tissue, 4T1 cells, human placenta tissue, human brain tissue, mouse cerebellum tissue, MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, human testis tissue, human lung cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ARPC4 Ready-To-Use IHC Kit. ARPC4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
10930-1-AP targets ARPC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, bovine |
| 免疫原 | ARPC4 fusion protein Ag1366 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin related protein 2/3 complex, subunit 4, 20kDa |
| 别名 | ARC20, Arp2/3 complex 20 kDa subunit, ARPC4, p20 Arc |
| 计算分子量 | 20 kDa |
| 观测分子量 | 19-25 kDa |
| GenBank蛋白编号 | BC012596 |
| 基因名称 | ARPC4 |
| Gene ID (NCBI) | 10093 |
| RRID | AB_2059951 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P59998 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ARP2/3 complex consists of seven subunits: two actin-related proteins (ARP2 and ARP3) and five ARP2/3 complex polypeptides (ARPC1B and ARPC2-5). ARPC4 is a core subunit of the actin-related protein (ARP2/3) complex, which catalyzes the formation of F-actin networks. ARPC4 is essential for the structural integrity of the ARP2/3 complex function. Mutations in ARPC4 cause Developmental delay, language impairment, and ocular abnormalities (DEVLO)(PMID: 35047857).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARPC4 antibody 10930-1-AP | Download protocol |
| IHC protocol for ARPC4 antibody 10930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Alliance A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells | ||
Biochim Biophys Acta Proteins Proteom Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis. | ||
Drug Resist Updat Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane | ||
Biomolecules Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model |
