验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, mouse brain, rat brain |
| Positive IHC detected in | human colon cancer tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ARPC3 Ready-To-Use IHC Kit. ARPC3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14652-1-AP targets ARPC3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, bovine |
| 免疫原 |
CatNo: Ag6249 Product name: Recombinant human ARPC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-178 aa of BC067747 Sequence: MPAYHSSLMDPDTKLIGNMALLPIRSQFKGPAPRETKDTDIVDEAIYYFKANVFFKNYEIKNEADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFPLNAIYAKPANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWWTCFVKRQFMNKSLSGPGQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin related protein 2/3 complex, subunit 3, 21kDa |
| 别名 | ARC21, Arp2/3 complex 21 kDa subunit, ARPC3 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC067747 |
| 基因名称 | ARPC3 |
| Gene ID (NCBI) | 10094 |
| RRID | AB_2059933 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15145 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARPC3 antibody 14652-1-AP | Download protocol |
| WB protocol for ARPC3 antibody 14652-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. | ||
J Cell Biol α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.
| ||
EMBO Rep WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis. | ||
Cell Rep Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesis
| ||
Biomolecules Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model | ||
Biomolecules Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis |
