验证数据展示

WB analysis using 15058-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARPC2 antibody included
IHC staining of human breast cancer using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARPC2 antibody included
IF Staining of HeLa using 15058-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARPC2 antibody (15058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 15058-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (15058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
WB analysis of MCF-7 using 15058-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15058-1-AP
IP result of anti-ARPC2 (IP:15058-1-AP, 4ug; Detection:15058-1-AP 1:6000) with HeLa cells lysate 1200 ug.
IHC staining of human colon using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARPC2 antibody included
IHC staining of mouse brain using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARPC2 antibody included

Featured Product
KD/KO验证

ARPC2 Polyclonal antibody

ARPC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15058-1-AP

产品说明书

引用文献(10)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

Actin-related protein 2/3 complex subunit 2, ARC34, p34 Arc, p34-ARC

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (10)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HT-1080 cells, HeLa cells, mouse skeletal muscle tissue, PC-3 cells, 3T3-L1 cells, NIH/3T3 cells, MCF-7 cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human colon tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy ARPC2 Ready-To-Use IHC Kit. ARPC2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 8 publications below
IF	See 1 publications below
IP	See 1 publications below

产品信息

15058-1-AP targets ARPC2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IF, IP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	CatNo: Ag7041 Product name: Recombinant human ARPC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC000590 Sequence: MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	actin related protein 2/3 complex, subunit 2, 34kDa
别名	Actin-related protein 2/3 complex subunit 2, ARC34, p34 Arc, p34-ARC
计算分子量	34 kDa
观测分子量	34 kDa
GenBank蛋白编号	BC000590
基因名称	ARPC2
Gene ID (NCBI)	10109
RRID	AB_2059787
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	O15144
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
FC protocol for ARPC2 antibody 15058-1-AP	Download protocol
IF protocol for ARPC2 antibody 15058-1-AP	Download protocol
IHC protocol for ARPC2 antibody 15058-1-AP	Download protocol
IP protocol for ARPC2 antibody 15058-1-AP	Download protocol
WB protocol for ARPC2 antibody 15058-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Dev Cell Aberrant cortex contractions impact mammalian oocyte quality Authors - Elvira Nikalayevich View Article
human	WB	Dev Cell Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages Authors - Xinyi Huang View Article
	WB	Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein Authors - Megan L Norris View Article
human	WB	J Cell Biol α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3. Authors - Nikki R Paul KD Validated View Article
human	IP	EMBO Rep WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis. Authors - Kailiang Zhao View Article
human	WB	J Neurosci The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse. Authors - Avanti Gokhale View Article

验证数据展示

WB analysis using 15058-1-AP

IHC staining of human breast cancer using 15058-1-AP

IHC staining of human breast cancer using 15058-1-AP

IF Staining of HeLa using 15058-1-AP

FC experiment of HeLa using 15058-1-AP

WB analysis of MCF-7 using 15058-1-AP

IP experiment of HeLa using 15058-1-AP

IHC staining of human colon using 15058-1-AP

IHC staining of mouse brain using 15058-1-AP

ARPC2 Polyclonal antibody

ARPC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

Aberrant cortex contractions impact mammalian oocyte quality

Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein

α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.

WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.

The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse.