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ARNTL Polyclonal antibody
ARNTL Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IHC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, 3T3-L1 cells, HeLa cells, MCF-7 cells, NCI-H1299 cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy BMAL1/ARNTL Ready-To-Use IHC Kit. BMAL1/ARNTL primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14268-1-AP targets ARNTL in WB, IP, IF, FC, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC, CoIP, chIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | ARNTL fusion protein Ag5586 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | aryl hydrocarbon receptor nuclear translocator-like |
| Synonyms | ARNT3, ARNTL, bHLH PAS protein JAP3, bHLHe5, BMAL1, BMAL1c, Brain and muscle ARNT like 1, JAP3, Member of PAS protein 3, MOP3, PASD3, TIC |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 69-75 kDa |
| GenBank Accession Number | BC041129 |
| Gene Symbol | ARNTL |
| Gene ID (NCBI) | 406 |
| RRID | AB_2878037 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00327 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARNTL antibody 14268-1-AP | Download protocol |
| IHC protocol for ARNTL antibody 14268-1-AP | Download protocol |
| IP protocol for ARNTL antibody 14268-1-AP | Download protocol |
| FC protocol for ARNTL antibody 14268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. | ||
J Pineal Res Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors | ||
Sci Total Environ Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario | ||
Theranostics Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia. | ||
Environ Pollut Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction. |
