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  • Featured Product
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ARNT2 Polyclonal antibody

ARNT2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 12810-1-AP

Print datasheet

Synonyms

ARNT protein 2, ARNT2, bHLHe1, KIAA0307

验证数据展示

  • WB analysis of Jurkat using 12810-1-AP

    WB analysis of Jurkat using 12810-1-AP

    WB result of ARNT2 antibody (12810-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT2 transfected Jurkat cells.

  • IHC staining of human gliomas using 12810-1-AP

    IHC staining of human gliomas using 12810-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12810-1-AP (ARNT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 12810-1-AP

    WB analysis of HEK-293 using 12810-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12810-1-AP targets ARNT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ARNT2 fusion protein Ag3536 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aryl-hydrocarbon receptor nuclear translocator 2
Synonyms ARNT protein 2, ARNT2, bHLHe1, KIAA0307
Calculated Molecular Weight 716 aa, 79 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC036099
Gene Symbol ARNT2
Gene ID (NCBI) 9915
RRIDAB_2059456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aryl-hydrocarbon receptor nuclear translocator 2(ARNT2), is a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. It specifically recognizes the xenobiotic response element(XRE). ARNT2 plays an important role in normal glucose handling in pancreatic beta cell function in humans and mice. ARNT2 proteins are dimeric partners for other PAS proteins such as HIF and SIM. ARNT2 and the other ARNT family members, e.g., ARNT, hypoxin inducible factor 1α (HIF1α), HIF2α, and aryl hydrocarbon receptor (AhR), are thought to function as heterodimers, and regulate the expression, and thereby the function, of many genes. Under hypoxic conditions, it complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. Modulation of ARNT2 in pancreatic beta cells affects glucose stimulated INS secretion (GSIS). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human ARNT2.

实验方案

Product Specific Protocols
WB protocol for ARNT2 antibody 12810-1-APDownload protocol
IHC protocol for ARNT2 antibody 12810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Microbiol

Fine Particulate Matter (PM2.5) Promotes CD146 Expression in Alveolar Epithelial Cells and Cryptococcus neoformans Pulmonary Infection.

Authors - Zhixiao Sun
View Article