验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Calu-1 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14105-1-AP targets ARNT/HIF-1 beta in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag5238 Product name: Recombinant human ARNT,HIF1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC060838 Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aryl hydrocarbon receptor nuclear translocator |
| 别名 | ARNT, HIF-1 beta, HIF1B, bHLHe2, Class E basic helix-loop-helix protein 2 |
| 计算分子量 | 416 aa, 46 kDa, 87 kDa |
| 观测分子量 | 87 kDa |
| GenBank蛋白编号 | BC060838 |
| 基因名称 | ARNT/HIF-1 beta |
| Gene ID (NCBI) | 405 |
| RRID | AB_10639523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P27540 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
| IHC protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
| IP protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
| WB protocol for ARNT/HIF-1 beta antibody 14105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
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