验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse placenta tissue, mouse retina tissue, rat retina tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28001-1-AP targets ARMC9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag27511 Product name: Recombinant human ARMC9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 188-537 aa of BC004514 Sequence: LDYEKLKKDLILGSDRLKAFLLQALRWRLTTSHPGEQRETVLQAYISNDLLDCYSHNQRSVLQLLHSTSDVVRQYMARLINAFASLAEGRLYLAQNTKVLQMLEGRLKEEDKDIITRENVLGALQKFSLRRPLQTAMIQDGLIFWLVDVLKDPDCLSDYTLEYSVALLMNLCLRSTGKNMCAKVAGLVLKVLSDLLGHENHEIQPYVNGALYSILSVPSIREEARAMGMEDILRCFIKEGNAEMIRQIEFIIKQLNSEELPDGVLESDDDEDEDDEEDHDIMEADLDKDELIQPQLGELSGEKLLTTEYLGIMTNTGKTRRKGLANVQWSGDEPLQRPVTPGGHRNGYPV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | armadillo repeat containing 9 |
| 别名 | Armadillo repeat-containing protein 9, KIAA1868, LisH domain-containing protein ARMC9, Melanoma/melanocyte-specific tumor antigen KU-MEL-1, NS21 |
| 计算分子量 | 817 aa, 92 kDa |
| 观测分子量 | 76 kDa |
| GenBank蛋白编号 | BC004514 |
| 基因名称 | ARMC9 |
| Gene ID (NCBI) | 80210 |
| RRID | AB_3669633 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z3E5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARMC9 (Armadillo Repeat Containing 9) belongs to the ARMC subfamily characterized by a class of tandem repeat proteins with a length of approximately 42 amino acids (PMID: 34912852). ARMC9 localizes to centrioles and the proximal portion of the cilium in mammalian cilia (PMID: 32453716). The variants of ARMC9 lead to Joubert syndrome (JBTS) ,a recessive neurodevelopmental ciliopathy characterized by characterized by hypotonia, ataxia, abnormal eye movements, and variable cognitive impairment(PMID: 28625504; PMID: 32453716). ARMC9 has two isoforms with molecular weights of 92 kDa and 76 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ARMC9 antibody 28001-1-AP | Download protocol |
| WB protocol for ARMC9 antibody 28001-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
