验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, HEK-293 cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat heart tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20506-1-AP targets ARMC10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | ARMC10 fusion protein Ag14313 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | armadillo repeat containing 10 |
| 别名 | armadillo repeat containing 10, Armadillo repeat-containing protein 10, PNAS 112, PSEC0198, SVH |
| 计算分子量 | 343 aa, 38 kDa |
| 观测分子量 | 31-37 kDa |
| GenBank蛋白编号 | BC003586 |
| 基因名称 | ARMC10 |
| Gene ID (NCBI) | 83787 |
| RRID | AB_10696176 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N2F6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARMC10 antibody 20506-1-AP | Download protocol |
| IHC protocol for ARMC10 antibody 20506-1-AP | Download protocol |
| IF protocol for ARMC10 antibody 20506-1-AP | Download protocol |
| IP protocol for ARMC10 antibody 20506-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis The Armc10/SVH gene: genome context, regulation of mitochondrial dynamics and protection against Aβ-induced mitochondrial fragmentation. | ||
Heliyon Prognostic value and immune infiltration of ARMC10 in pancreatic adenocarcinoma via integrated bioinformatics analyses
| ||
J Cell Mol Med ARMC10 regulates mitochondrial dynamics and affects mitochondrial function via the Wnt/β-catenin signalling pathway involved in ischaemic stroke
| ||
BMC Cancer Comprehensive analysis of the expression and prognostic value of ARMCs in pancreatic adenocarcinoma |
