验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HepG2 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17060-1-AP targets ARL8A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10788 Product name: Recombinant human ARL8A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC015408 Sequence: MIALFNKLLDWFKALFWKEEMELTLVGLQYSGKTTFVNVIASGQFNEDMIPTVGFNMRKITKGNVTIKLWDIGGQPRFRSMWERYCRGVSAIVYMVDAADQEKIEASKNELHNLLDKPQLQGIPVLVLGNKRDLPGALDEKELIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADP-ribosylation factor-like 8A |
| 别名 | GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B |
| 计算分子量 | 186 aa, 21 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC015408 |
| 基因名称 | ARL8A |
| Gene ID (NCBI) | 127829 |
| RRID | AB_2058998 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96BM9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A has a calculated molecular weight of 21 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARL8A antibody 17060-1-AP | Download protocol |
| IP protocol for ARL8A antibody 17060-1-AP | Download protocol |
| WB protocol for ARL8A antibody 17060-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy | ||
Nat Commun RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. | ||
Curr Biol ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1. | ||
J Cell Biol Specific KIF1A-adaptor interactions control selective cargo recognition.
| ||
J Cell Sci The Salmonella effector SifA initiates a kinesin-1 and kinesin-3 recruitment process mirroring that mediated by Arl8a and Arl8b.
| ||
