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验证数据展示

  • WB analysis using 10961-1-AP

    WB analysis using 10961-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of IMCD3 cells using 10961-1-AP

    IF Staining of IMCD3 cells using 10961-1-AP

    IF result of 10961-1-AP (anti-ARL3,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus).

  • WB analysis of HeLa using 10961-1-AP

    WB analysis of HeLa using 10961-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 10961-1-AP

    IHC staining of human testis using 10961-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ARL3 antibody included
  • IHC staining of human testis using 10961-1-AP

    IHC staining of human testis using 10961-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ARL3 antibody included
  • IF Staining of hTERT-RPE1 using 10961-1-AP

    IF Staining of hTERT-RPE1 using 10961-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MDCK using 10961-1-AP

    IF Staining of MDCK using 10961-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

ARL3 Polyclonal antibody

ARL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10961-1-AP

产品说明书

引用文献(21)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

ADP ribosylation factor like 3, ADP-ribosylation factor-like protein 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, PC-3 cells, human brain tissue, mouse testis tissue, rat brain tissue, HeLa cells, rat testis tissue
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells, IMCD3 cells, hTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy ARL3 Ready-To-Use IHC Kit. ARL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10961-1-AP targets ARL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse
免疫原

CatNo: Ag1406

Product name: Recombinant human ARL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-182 aa of BC009841

Sequence: MGLLSILRKLKSAPDQEVRILLLGLDNAGKTTLLKQLASEDISHITPTQGFNIKSVQSQGFKLNVWDIGGQRKIRPYWKNYFENTDILIYVIDSADRKRFEETGQELAELLEEEKLSCVPVLIFANKQDLLTAAPASEIAEGLNLHTIRDRVWQIQSCSALTGEGVQDGMNWVCKNVNAKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 3
别名 ADP ribosylation factor like 3, ADP-ribosylation factor-like protein 3
计算分子量 20 kDa
观测分子量 20 kDa, 25 kDa
GenBank蛋白编号BC009841
基因名称 ARL3
Gene ID (NCBI) 403
RRIDAB_2274220
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36405
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

实验方案

Product Specific Protocols
IF protocol for ARL3 antibody 10961-1-APDownload protocol
IHC protocol for ARL3 antibody 10961-1-APDownload protocol
WB protocol for ARL3 antibody 10961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

A stress-induced cilium-to-PML-NB route drives senescence initiation

Authors - Xiaoyu Ma
View Article
humanWB,IF

Nat Commun

Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation.

Authors - Antonios D Konitsiotis
View Article
mouseWB,IP,IF

Nat Commun

Arl3 and LC8 regulate dissociation of dynactin from dynein.

Authors - Mingyue Jin
  • KD Validated
View Article
mouseWB

Nat Commun

Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

Authors - Hyunho Kim
  • KD Validated
View Article
humanWB

Proc Natl Acad Sci U S A

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Authors - Humbert Melissa C MC
View Article
humanIF

Dev Cell

The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK.

Authors - Louise A Stephen
View Article
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