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ARID3A Monoclonal antibody

ARID3A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B10C10

Cat No : 68428-1-Ig

Print datasheet

Synonyms

ARID3A, BRIGHT, Dead ringer like protein 1, DRIL1, DRIL3, DRX, E2F binding protein 1, E2FBP1

验证数据展示

  • WB analysis using 68428-1-Ig

    WB analysis using 68428-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HuH-7 cells, Jurkat cells, K-562 cells, TF-1 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARID3A Ready-To-Use IHC Kit. ARID3A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68428-1-Ig targets ARID3A in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen ARID3A fusion protein Ag7655 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name AT rich interactive domain 3A (BRIGHT-like)
Synonyms ARID3A, BRIGHT, Dead ringer like protein 1, DRIL1, DRIL3, DRX, E2F binding protein 1, E2FBP1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC060828
Gene Symbol ARID3A
Gene ID (NCBI) 1820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99856
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).

实验方案

Product Specific Protocols
WB protocol for ARID3A antibody 68428-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols