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- KD/KO Validated
ARID3A Polyclonal antibody
ARID3A Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse placenta tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ARID3A Ready-To-Use IHC Kit. ARID3A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14068-1-AP targets ARID3A in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, ChIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | ARID3A fusion protein Ag5124 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | AT rich interactive domain 3A (BRIGHT-like) |
| Synonyms | ARID3A, BRIGHT, Dead ringer like protein 1, DRIL1, DRIL3, DRX, E2F binding protein 1, E2FBP1 |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC060828 |
| Gene Symbol | ARID3A |
| Gene ID (NCBI) | 1820 |
| RRID | AB_2060390 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99856 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARID3A antibody 14068-1-AP | Download protocol |
| IHC protocol for ARID3A antibody 14068-1-AP | Download protocol |
| IF protocol for ARID3A antibody 14068-1-AP | Download protocol |
| IP protocol for ARID3A antibody 14068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
PLoS Genet Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2. | ||
Carcinogenesis ARID3A promotes the development of colorectal cancer by upregulating AURKA.
| ||
Ann Surg Oncol High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma. | ||
Cell Biol Int ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells
| ||
