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验证数据展示

  • WB analysis using 23406-1-AP

    WB analysis using 23406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23406-1-AP (ARID2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 23406-1-AP

    IHC staining of mouse testis using 23406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23406-1-AP (ARID2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 23406-1-AP

    IHC staining of mouse testis using 23406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23406-1-AP (ARID2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARID2 Polyclonal antibody

ARID2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23406-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Zinc finger protein with activation potential, KIAA1557, BRG1-associated factor 200, BAF200, AT-rich interactive domain-containing protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23406-1-AP targets ARID2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag19968

Product name: Recombinant human ARID2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1533-1835 aa of BC090062

Sequence: VGVRIVTISDPNNAGCSATMVAVPAGADPSTVAKVAIESAVQQKQQHPPTYVQNVVPQNTPMPPSPAVQVQGQPNSSQPSPFSGSSQPGDPMRKPGQNFMCLWQSCKKWFQTPSQVFYHAATEHGGKDVYPGQCLWEGCEPFQRQRFSFITHLQDKHCSKDALLAGLKQDEPGQAGSQKSSTKQPTVGGTSSTPRAQKAIVNHPSAALMALRRGSRNLVFRDFTDEKEGPITKHIRLTAALILKNIGKYSECGRRLLKRHENNLSVLAISNMEASSTLAKCLYELNFTVQSKEQEKDSEMLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AT rich interactive domain 2 (ARID, RFX-like)
别名 Zinc finger protein with activation potential, KIAA1557, BRG1-associated factor 200, BAF200, AT-rich interactive domain-containing protein 2
计算分子量 1835 aa, 197 kDa
观测分子量200-230 kDa
GenBank蛋白编号BC090062
基因名称 ARID2
Gene ID (NCBI) 196528
RRIDAB_2918080
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ68CP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ARID2 antibody 23406-1-APDownload protocol
WB protocol for ARID2 antibody 23406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Am Heart Assoc

Aging-Associated ALKBH5-m6A Modification Exacerbates Doxorubicin-Induced Cardiomyocyte Apoptosis Via AT-Rich Interaction Domain 2

Authors - Runtai Chen
View Article
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