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验证数据展示

  • IF Staining of HepG2 using 82979-1-RR (same clone as 82979-1-PBS)

    IF Staining of HepG2 using 82979-1-RR (same clone as 82979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARID1B antibody (82979-1-RR, Clone: 230222G4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82979-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 82979-1-RR (same clone as 82979-1-PBS)

    FC experiment of HEK-293 using 82979-1-RR (same clone as 82979-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-HUMAN ARID1B (82979-1-RR, Clone:230222G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP)(blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82979-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82979-1-RR

    Affinity and Kinetic Characterization of 82979-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82979-1-RR against Human ARID1B were performed. The affinity constant is 0.606 nM.

ARID1B Recombinant antibody, PBS Only

ARID1B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82979-1-PBS

产品说明书

CloneNo. 230222G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ARID1B Ready-To-Use IHC Kit. ARID1B primary antibody included.

产品信息

82979-1-PBS targets ARID1B in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 ARID1B fusion protein Ag30643 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AT rich interactive domain 1B (SWI1-like)
别名 hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b
计算分子量 236 kDa
GenBank蛋白编号NM_017519
基因名称 ARID1B
Gene ID (NCBI) 57492
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NFD5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C