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验证数据展示

  • WB analysis using 30304-1-AP (same clone as 30304-1-PBS)

    WB analysis using 30304-1-AP (same clone as 30304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30304-1-AP (ARID1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 30304-1-AP (same clone as 30304-1-PBS)

    IP experiment of HeLa using 30304-1-AP (same clone as 30304-1-PBS)

    IP result of anti-ARID1A (IP:30304-1-AP, 4ug; Detection:30304-1-AP 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

    IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARID1A antibody included
  • IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

    IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARID1A antibody included
  • IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

    IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARID1A antibody included
  • IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

    IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARID1A antibody included

ARID1A Polyclonal antibody, PBS Only

ARID1A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 30304-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

BM029, BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ARID1A Ready-To-Use IHC Kit. ARID1A primary antibody included.

产品信息

30304-1-PBS targets ARID1A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30749

Product name: Recombinant human ARID1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1230-1370 aa of NM_006015

Sequence: KDPYGSMRKAPGSDPFMSSGQGPNGGMGDPYSRAAGPGLGNVAMGPRQHYPYGGPYDRVRTEPGIGPEGNMSTGAPQPNLMPSNPDSGMYSPSRYPPQQQQQQQQRHDSYGNQFSTQGTPSGSPFPSQQTTMYQQQQQNYK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AT rich interactive domain 1A (SWI-like)
别名 BM029, BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A
计算分子量 242 kDa
观测分子量250-260 kDa
GenBank蛋白编号NM_006015
基因名称 ARID1A
Gene ID (NCBI) 8289
RRIDAB_3086292
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14497
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.

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